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Two - phase cultivation of insect cells for production of recombinant protein

✍ Scribed by Sung Hwan Lee; Tai Hyun Park

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
396 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

Two-phase cultivation of insect cells was carried out to produce recombinant pgalactosidase and evaluate factors influencing high cell density and high expression. The primary factor limiting specific expression at high cell concentration was not the cell concentration but the medium condition. The addition of 4 g/L yeastolate during the growth phase increased cell density while the addition of 0.6 g/L glutamine in the production phase raised the specific expression rate. Productivity of recombinant protein was consequently improved seven-fold by this operation.

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✍ Lynn McCarroll; Linda A King πŸ“‚ Article πŸ“… 1997 πŸ› Elsevier Science 🌐 English βš– 594 KB

Insect cells are relatively cheap to maintain and are capable of producing accurately translated and correctly processed heterologous proteins. Recent research has focused on the development of improved expression vectors for continuous, high-level production of foreign proteins, including a number