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Two isoforms of ferredoxin:NADP+oxidoreductase from wheat leaves: purification and initial biochemical characterization

✍ Scribed by Joanna Grzyb; Przemysław Malec; Izabela Rumak; Maciej Garstka; Kazimierz Strzałka

Publisher
Springer
Year
2008
Tongue
English
Weight
477 KB
Volume
96
Category
Article
ISSN
0166-8595

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Homogeneous phosphoribulokinase (PRK ; ATP : D-ribulose-5-phosphate 1 -phosphotransferase, EC 2.7.1.19) was isolated from wheat leaves with a specific activity of 15 pkat mg-' protein. The purification included ammonium sulfate cuts, isoelectric precipitation, and hydrophobic and affinity chromatogr