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Two Fluorometric Approaches to the Measurement of Dextranase Activity

โœ Scribed by Mingjie Zhou; Cailan Zhang; Rosalyn H. Upson; Richard P. Haugland

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
68 KB
Volume
260
Category
Article
ISSN
0003-2697

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A fluorometric procedure for quantitating the amount of N-acetylneuraminic acid enzymatically released by the neuraminidase activity from N-acetylneuraminyl-lactose (sialyl-lactose) has been developed. The liberated lactose is hydrolyzed with beta-galactosidase, and the released galactose is oxidize