Two Fibrin Zymography Methods for Analysis of Plasminogen Activators on Gels

✍ Scribed by Nack-Shick Choi; Seung-Ho Kim

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 71 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4572

✦ Synopsis

smaller amounts of enzyme (data not shown). In addition, the two HoPL isoforms were detected both on native PAGE (silver nitrate staining) as well as on the corresponding zymogram as shown in Fig. 2.

In conclusion, the results of the present study show that the zymographic technique is a reliable method which can be used to perform rapid qualitative assessments on biological materials containing pancreatic lipase activity. This technique has several advantages, including its simplicity, sensitivity, specificity, and its speed of execution. We have not yet performed zymograms with lipases other than from pancreatic origin. However, this technique cannot be used indiscriminately with all lipases, since some of them hydrolyze resorufin ester rather poorly (8).

Acknowledgments. The authors are grateful to Dr. A. de Caro and J. de Caro (LLE-Marseille) for their generous gift of human pancreatic juice and purified HPL, to Dr. L. Dupuis and Dr. F. Beisson (LLE-Marseille) for helpful discussions, and to Dr. J. Blanc for revising the English. This research was carried out with the financial support of the EC FAIR 97 3228 project of the European Union.