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Two-dimensional spectra of intact tissue: Homonuclear Hartmann-Hahn spectroscopy provides increased sensitivity and information content as compared to COSY

✍ Scribed by Juli Alonso; Carles Arüs; William M. Westler; John L. Markley


Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
493 KB
Volume
15
Category
Article
ISSN
0740-3194

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✦ Synopsis


Abstract

Hartmann‐Hahn (HOH AHA) spectroscopy led to good definition of proton spin systems of metabolites such as lactate, (phospho) creatine, and carnosine from a 3‐g sample of excised frog gastrocnemius muscle in a 17‐min experiment. The sign of the HOHAHA cross‐peak intensity appears to provide additional information about interactions between metabolites and their protein binding sites. © 1990 Academic Press, Inc.