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Two-dimensional separations of peptides and proteins by comprehensive liquid chromatography-capillary electrophoresis

✍ Scribed by John P. Larmann Jr.; Anthony V. Lemmo; Alvin W. Moore Jr.; Professor James W. Jorgenson


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
969 KB
Volume
14
Category
Article
ISSN
0173-0835

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Comprehensive two-dimensional separation
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## Abstract A comprehensive two‐dimensional (2‐D) separation system, coupling capillary reverse‐phase liquid chromatography (cRPLC) to capillary isoelectric focusing (CIEF), is described for protein and peptide mapping. cRPLC, the first dimension, provided high‐resolution separations for salt‐free