Two-dimensional mid-IR and near-IR corre
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Christian P. Schultz; Heinz Fabian; Henry H. Mantsch
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Article
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1998
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John Wiley and Sons
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English
⚖ 354 KB
We introduce near-IR spectroscopy as an ancillary tool for monitoring structural changes of proteins in aqueous solution using ribonuclease A (RNase A) as a model protein. The thermal unfolding of RNase A results in clear spectral changes in the near-IR and the mid-IR regions. In the near-IR the mos