Two-dimensional map of human brain proteins

✍ Scribed by Hanno Langen; Peter Berndt; Daniel Röder; Nigel Cairns; Gert Lubec; Michael Fountoulakis

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 841 KB
• Volume: 20
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/(sici)1522-2683(19990101)20:4/5<907::aid-elps907>3.0.co;2-a

✦ Synopsis

Samples of human brain from the parietal cortex lobe were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradient strips covering the various pH regions. The protein spots were visualized with colloidal Coomassie blue stain and identified by matrix-assisted laser desorption/ionization mass spectrometry. Approximately 400 spots were identified, corresponding to 180 different brain proteins. The list of identified proteins includes a large number of structural proteins and of enzymes or enzyme subunits with various catalytic activities. The majority of proteins are localized in the cytoplasma and in mitochondria. The two-dimensional map may be useful as a reference database to study changes in the protein level caused by various disorders, such as Alzheimer©s disease, major depression and schizophrenia.