𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Two-dimensional gel electrophoresis separation and N-terminal sequence analysis of proteins from Clostridium pasteurianum W5

✍ Scribed by Dr. Ragnar Flengsrud; Lars Skjeldal


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
492 KB
Volume
19
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

✦ Synopsis


Abstract

Proteins from Clostridium pasteurianum were separated by two‐dimensional gel electrophoresis. Amino‐terminal sequence determination and sequence analysis allowed the identification of 20 proteins, while 11 protein sequences remained unidentified and one protein appeared to have a blocked amino terminus.


πŸ“œ SIMILAR VOLUMES


Capillary electrophoresis/tandem mass sp
✍ Xiaoying Jin; Yajuan Chen; David M. Lubman; David Misek; Samir M. Hanash πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 102 KB πŸ‘ 2 views

Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip

Analysis of proteins from membrane-enric
✍ Giulia Friso; Lilian WikstrΓΆm πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 183 KB πŸ‘ 2 views

Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the