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Two-dimensional electrophoretic analysis of Corynebacterium glutamicum membrane fraction and surface proteins

✍ Scribed by Thomas Hermann; Melanie Finkemeier; Walter Pfefferle; Gregor Wersch; Reinhard Krämer; Andreas Burkovski


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
215 KB
Volume
21
Category
Article
ISSN
0173-0835

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Two-dimensional electrophoresis of proteins is often precluded due to the lack of solubilization of cell membrane extracts in an aqueous medium. Various additives and detergents have been used to circumvent the problem, but their efficacy may not be satisfactory. In this study, the removal of lipidi