Two-dimensional electrophoresis and peptide mass fingerprinting of bacterial outer membrane proteins
β Scribed by Mark P. Molloy; Nikhil D. Phadke; Janine R. Maddock; Philip C. Andrews
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 146 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0173-0835
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Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the
Two-dimensional electrophoresis of proteins is often precluded due to the lack of solubilization of cell membrane extracts in an aqueous medium. Various additives and detergents have been used to circumvent the problem, but their efficacy may not be satisfactory. In this study, the removal of lipidi