Two-dimensional electrophoresis: An application combining cellulose acetate mambrane with SDS-polyacrylamide gradient gel
✍ Scribed by R.J. Blagrove; M.J. Frenkel
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 988 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Two-Dimensional
Electrophoresis: An Application Combining Cellulose Acetate Membrane with SDS-Polyacrylamide Gradient Gel
A simple, effective method for high-resolution two-dimensional electrophoresis is described. The combination of cellulose acetate with SDS-polyacrylamide gradient gels has been studied for a range of standard proteins and for comparison of lupine seed storage proteins. The speed of the electrophoretic separation and the general ease of handling of cellulose acetate membranes make them ideal for the first dimension. Furthermore, because these membranes are very thin, reduction with P-mercaptoethanol and incubation with SDS can be achieved immediately on top of the second-dimension slab.