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Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics

✍ Scribed by Arruda, Sandra Cristina Capaldi; Barbosa, Herbert de Sousa; Azevedo, Ricardo Antunes; Arruda, Marco Aurélio Zezzi

Book ID
120422099
Publisher
Royal Society of Chemistry
Year
2011
Tongue
English
Weight
276 KB
Volume
136
Category
Article
ISSN
0003-2654

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Two-dimensional polyacrylamide gel electrophoresis (2-DE) and mass spectrometry are being used as proteomic tools in an integrated functional genomics program focused on the model legume Medicago truncatula. Due to the perceived high levels of indeterminate error associated with 2-DE we deemed it ne