Two-color analysis of peripheral lymphocyte surface antigens in inherently healthy adults

✍ Scribed by Masayoshi Yamashiki; Akira Nishimura; Yoshitane Kosaka; Stephen P. James

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 432 KB
• Volume: 8
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860080106

✦ Synopsis

We studied 134 "healthy persons" with no past history of any serious or latent diseases, and observed differences in their 25 lymphocyte subsets by conducting two-color analyses using 12 useful combinations of dye-labeled monoclonal antibodies.

CD4+Leu8+ cells and CD25+CD3+ Celts were significantly higher in males than in females, whereas CD4+Leu8-cells and CD23+CD19+ cells were significantly higher in females. In comparison ofthe 25 lymphocyte subsets among four age groups, CD45RA-CD4+ cetls and CDw29+CD4+ cells significantly increased with age, and CD8+ CD11 b-cells and CD57-CD8+ cells significantly decreased with age.

In females, helper T cells significantly increased and helper inducer T cells increased, while cytotoxic T cells decreased with age. These are important findings that should be considered in studies of immune function and autoimmune disorders. o I ~~~W I ~~Y -L I S S .