Two cDNA clones coding for the legumin protein ofPisum sativumL. contain sequence repeats
✍ Scribed by Grantley W. Lycett; Ashton J. Delauney; Wenming Zhao; John A. Gatehouse; Ronald R. D. Croy; Donald Boulter
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 434 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
The sequence of two cDNA clones coding for the whole of the fl-subunit and most of the a-subunit of legumin are presented together with a considerable amount of protein sequence data to confirm the predicted amino acid sequence. A unique feature shown by these cDNAs is the presence of three 56 base pair tandem repeats in the region encoding the C terminal of the c~ polypeptide. The tandem repeats are also exhibited in the predicted polypeptide sequence as three 18 amino acid repeats which contain extremely high proportions of polar, mainly acidic, residues. The new sequences are compared to the previously published sequence of some shorter legumin cDNAs (Nature 295: 76-79). In the region where the sequences overlap, the previous cDNAs differ from the new ones by only a few base substitutions but most of the repeated region is not present though the sequences on either side are. The possibility that the absence of the repeats may reflect the difference between two types of legumin gene, rather than an artefact of the cloning of the cDNAs, is discussed.