Turnover of protein components of the plasma membrane of Saccharomyces cerevisiae
β Scribed by Enrique Herrero; Javier Pastor; Rafael Sentandreau
- Book ID
- 115725574
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 558 KB
- Volume
- 689
- Category
- Article
- ISSN
- 0005-2736
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π SIMILAR VOLUMES
1. The distribution of ATPase and several marker enzymes was examined after differential and sucrose gradient centrifugation of yeast homogenates. 2. An ATPase activity not sensitive to oligomycin is found exclusively associated with a particulate fraction equilibrating at densities of 1.23-1.25. Th
1. Plasma membrane preparations have been isolated from spheroplasts of Saccharomyces cerevisiae, strain R XII, via lysis and subsequent differential centrifugation. These preparations are almost devoid of mitochondrial contamination. 2. The plasma membrane ATPase is fairly stable when refrigerated,