Turbidimetric determination of blood aminoglycoside levels by growth curve analysis
✍ Scribed by Stephen C. Edberg; Abraham Miskin
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 231 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
A procedure to assay rapidly blood aminoglycoside levels was developed based on growth curve analysis of continuous absorbance measurements of a bacterial test strain. Of the means available for monitoring bacterial growth quantitatively, turhidimetric measurement was selected because it can be used to perform a clinical assay in <4 hr. Continuous turbidimetric measurements provide information as soon as the antimicrobial agent affects growth, which often occurs within 60 min of the start of an assay. Several bacterial isolates were evaluated before Staphylococcus aureus (MHMC 386) was chosen as the test microorganism. This isolate was quite sensitive to the aminoglycoside antibiotics, permitted the rapid measurement of blood levels of amikacin, gentamicin, kanamycin, and tohramycin, and exhibited a linear doseresponse relationship of turbidity over a wide range of antibiotic concentrations.
Keyphrases 0 Antibiotics, aminoglycoside-amikacin, gentamicin, kanamycin, and tobramycin, effect on bacterial growth, turbidimetric measurement of blood levels 0 Antibacterials-amikacin, gentamicin, kanamycin, and tobramycin, effect on bacterial growth, turbidimetric measurement of blood levels 0 Spectrophot.ometry-turbidimetric determination of blood levels of amikacin, gentamicin, kanamycin, and tobramycin, effect on bacterial growth
The concentration at which aminoglycoside antibiotics injure animal cells is only two to four times greater than the concentration required to inhibit most common bacterial pathogens. In addition, distribution of the drug to the various body compartments is not the same in all individuals. Accordingly, a rapid assay is required for the optimal usage of aminoglycosides.