Tunable optical setup with high flexibility for spectrally resolved coherent anti-Stokes Raman scattering microscopy
✍ Scribed by G. Bergner; D. Akimov; S. Schlücker; H. Bartelt; B. Dietzek; J. Popp
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 172 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1612-2011
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✦ Synopsis
A simplified setup for coherent anti-Stokes Raman scattering (CARS) microscopy is introduced, which allows for recording CARS images with 30 cm -1 excitation bandwidth for probing Raman bands between 500 and 900 cm -1 with minimal requirements for alignment. The experimental arrangement is based on electronic switching between CARS images recorded at different Raman resonances by combining a photonic crystal fiber (PCF) as broadband light source and an acousto-optical programmable dispersive filter (AOPDF) as tunable wavelength filter. Such spatial light modulator enables selection of a narrow-band spectrum to yield high vibrational contrast and hence chemical contrast in the resultant CARS images. Furthermore, an experimental approach to reconstruct spectral information from CARS image contrast is introduced.
Raman intensity, arb. units 0.6 0.4 0.