Tumours derived from HTLV-Itax transgenic mice are characterized by enhanced levels of apoptosis and oncogene expression
✍ Scribed by Hall, Anthony P.; Irvine, Jane; Blyth, Karen; Cameron, Ewan R.; Onions, David E.; Campbell, Moyra E. M.
- Book ID
- 102648471
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 582 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
In order to investigate the role that the human T-lymphotropic virus type I (HTLV-I) tax oncogene plays in apoptosis and transformation in vivo, four lines of HTLV-I tax transgenic mice were generated under the regulatory control of the CD3promoter-enhancer sequence. These mice develop a variety of phenotypes including mesenchymal tumours, which develop at wound sites, and salivary and mammary adenomas. In situ DNA fragment labelling and immunocytochemical analysis of these tumours reveals that they display enhanced levels of apoptosis, which is associated with elevated levels of Myc, Fos, Jun, and p53 protein expression. Furthermore, double immunofluorescent staining shows that Tax expression and apoptosis co-localize, indicating that Tax expression is closely associated with apoptosis in vivo.