Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines

✍ Scribed by Alicia V. Palleroni; Luigi Varesio; Rosemary B. Wright; Michael J. Brunda

• Book ID: 102869959
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 781 KB
• Volume: 49
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910490226

✦ Synopsis

Continuous alveolar macrophage (AM) and tumorinfiltrated (TIM) cell lines have been generated from C57816J mice by in vitro infection with the 12 retrovirus carrying the v-raf and v-myc oncogens. Four cloned AM cell lines (AMJZ-C8, AMJZ-C10, AMJZ-CI I, AMJZ-CZO) and 3 cloned TIM cell lines (TIMJZ-C4, TIMJ2-C7 and TIMJZ-C 15) were expanded for further characterization. Flow cytometry detected the product of the raf gene in the cytoplasm of all these cell lines. Studies on the tumoricidal properties of these AM and TIM cell lines demonstrated differences in their response to a panel of known macrophage activators. Four of these cell lines (AMJZ-C8, AMJ2-C10, TIMJZ-C7 and TIMJZ-CI 5) were activated following exposure to recombinant murine interferon gamma (rMulFN-y) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP). AMJZ-CZO was only activated by incubation with rMulFN-y plus LPS. AMJZ-CI I and TIMJZ-C4 are the cell lines that most closely resembled the response pattern of the parental AM and TIM, since they could be activated by either the combination of rMulFN-y plus LPS or rMulFN-y plus MDP. Constitutive expression of MHC-class-ll antigens was low on AMJZ-C I I or TIMJZ-C4 but was increased following exposure to rMulFN-y. Neither cell line secreted substantial amounts of IL-l or TNF but both secreted large amounts of IL-6. Thus these cell lines could be powerful tools to study AM and TIM activation and cytotoxicity.