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Tumor-selective cytotoxic effects of murine tumor necrosis factor (TNF) and interferon-gamma (EN-gamma) in organ culture of B16 melanoma cells and heart tissue

✍ Scribed by Marc Mareel; Christian Dragonetti; Jan Tavernier; Walter Fiers

Publisher: John Wiley and Sons
Year: 1988
Tongue: French
Weight: 806 KB
Volume: 42
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910420327

No coin nor oath required. For personal study only.

✦ Synopsis

Organotypically cultured, confronting pairs of 8 16816 cell clusters and fragments of embryonic chick or mouse heart, as used for the study of invasion in vitro, were treated with murine TNF plus IFN-gamma for 4 and 7 days. This treatment selectively killed the 816816 cells and left the heart tissue intact as assayed by histology and by plating confronting pairs and spent medium on tissue culture substrate. Using this organ culture assay, which more closely mimics the situation in vivo, we confirmed the tumor-selective cytotoxic effects of combined treatment with TNF and IFN-gamma as observed in separate cultures of malignant and non-malignant cells on "artificial" substrate.

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