Tumor regression and induction of anti-tumor immunity by local chemotherapy of guinea-pigs bearing a line-10 hepatocarcinoma
✍ Scribed by Anke M. E. Claessen; Henriëtte Valster; Herman Bril; Peter A. Steerenberg; Ben T. G. Tan; Rik J. Scheper
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- French
- Weight
- 903 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
MATERIAL AND METHODS
Animals
Sewall-Wright inbred strain-2 guinea-pigs were bred at the Department of Experimental Medicine (Free University, Amsterdam, The Netherlands). Animals of either sex, aged 3-6 months, weighing 300-500 g, were used for the various experiments. They were caged in groups of 6 and fed guinea-pig ration (Hope Farms, Woerden, The Netherlands) and water ad libitum.
Tumor
The line-10 and line-1 hepatocarcinomas were originally induced in strain-2 guinea-pigs by oral feeding with diethylnitrosamine and converted to an ascites form (Rapp et al., 1968). Tumor cells were collected routinely by peritoneal la-3To whom correspondence and reprint requests should be sent, at the