Tumor necrosis factor (TNF?) production by rat peritoneal macrophages is not polyacrylate surface-chemistry dependent
✍ Scribed by Ung, Derek Y. ;Woodhouse, Kimberly A. ;Sefton, Michael V.
- Book ID
- 101258362
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 164 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Polyacrylate films in the absence of added endotoxin caused rat peritoneal macrophages to secrete a small amount of TNF␣. There was little difference, if any, among the materials, which included various co-or ter-polymers of hydroxyethyl methacrylate, dimethylaminoethyl methacrylate, methacrylic acid, methyl methacrylate, and butyl methacrylate. The materials were surface characterized and endotoxin cleaned prior to testing. Equivalent endotoxin levels associated with the material were <0.03 EU/mL for all materials but two; for polyHEMA, the most contaminated material, it was 0.23 EU/mL. Films of the materials were incu-bated with freshly isolated rat peritoneal macrophages for 6 to 24 h before the TNF␣ levels in the supernatant were analyzed for biological activity, using L929 cells as a target. When endotoxin was added, far greater quantities of TNF␣ were generated at 24 h compared to 6 h, but still there was little effect with regard to material chemistry. Such an in vitro assay proved not to be useful for the screening of potential microencapsulation materials for peritoneal biocompatibility.