✦ LIBER ✦

Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea

✍ Scribed by Phillips, Gregg D. ;Stone, A. Marika ;Schultz, Julie C. ;Jones, Bryan D. ;Lisowski, Mark J. ;Goodkin, Margot L. ;Knighton, David R.

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 493 KB
Volume: 245
Category: Article
ISSN: 0003-276X
DOI: 10.1002/(sici)1097-0185(199605)245:1<53::aid-ar9>3.0.co;2-g

No coin nor oath required. For personal study only.

✦ Synopsis

Background:

The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF).

Methods: rhTNF (0.5 ng to 1.0 pg) was incorporated into the slow release polymers Hydron or HYPAN@ and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay.

Results: All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN@ released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated.

Conclusion: Under the circumstances tested, rhTNF is not angiogenic i n vivo. o 1996 Wiley-Liss, Inc.

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