𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. VIII. Expression and shedding of Fcγ receptors on metastatic tumor cell variants

✍ Scribed by Schirrmacher, Volker ;Jacobs, Wolfgang

Publisher
Wiley (John Wiley & Sons)
Year
1979
Tongue
English
Weight
687 KB
Volume
11
Category
Article
ISSN
0091-7419

No coin nor oath required. For personal study only.

✦ Synopsis

The expression of receptors for the Fc portion of IgG immunoglobin molecules was studied on tumor cell lines with high and low metastatic capacity. Two tumor cell lines from DBA/2 mice that had high metastatic activity, ESb and MDAY-D2, contained a high percentage of Fc receptor positive cells, as detected in a rosette assay with IgG antibody-coated erythrocytes (EA). In contrast, the low metastatic parental line Eb, from which ESb was derived, contained only a low percentage of EA-rosette-forming cells. ESb ascites tumor cells adapted t o tissue culture in the presence of 2mercaptoethanol (2ME) had a high expression of Fc receptors, whereas a cell line adapted t o tissue culture in the absence of 2ME had a low expression of Fc receptors.

t o cause blocking of rosette formation. They were found to be present in fluids from tumor-bearing animals, such as serum and cell-free ascites. Even animals with an ascites tumor of the low-metastatic line Eb contained "soluble" Fc receptors.

The results are discussed with regard t o their possible significance for tumor metastasis.

"Soluble" Fc receptors were detectable by their ability t o bind t o EA and

Key words: tumor metastases, Fc receptor, shedding, tumor variants

Properties of tumor cells that may be impor lant for tumor cell dissemination and formation of metastasis are investigated in a syngeneic tumor model system in DBA/2 mice. The system consists of a methylcholanthrene-induced lymphoma (Eb) with low metastatic potential and a spontaneous variant thereof (ESb) with high metastatic capability. An unrelated methylcholanthrene-induced tumor (MDAY-D2) with pronounced metastasizing capacity is included for comparison. The major characteristics of these tumor cells have already been described [ 1-41 and are summarized in Table I. We showed

📜 SIMILAR VOLUMES

Tumor metastases and cell-mediated immun
Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice X. Immunoselection of tumor variants differing in tumor antigen expression and metastatic capacity
✍ V. Schirrmacher; K. Bosslet 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 737 KB

## Abstract We previously described morphological, functional and antigenic differences between a chemically‐induced DBA/2 lymphoma, Eb, and a spontaneous variant, ESb, which arose in 1968 and had highly increased metastatic capacity. Now we present evidence that the two cell lines, in spite of the

Tumor metastases and cell-mediated immun
Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice.: Iv. Antigenic differences between a metastasizing variant and the parental tumor line revealed by cytotoxic T lymphocytes
✍ V. Schirrmacher; K. Bosslet; G. Shantz; K. Clauer; D. Hijbsch 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 797 KB

## Abstract The syngeneic cytotoxic T‐cell response against a metastasizing murine lymphoma variant was investigated and compared with the response against the non‐metastasizing parental tumor line Eb. Anti‐tumor cytotoxicity was not detectable in a 4‐h ^51^Cr release assay in spleens taken directl

Tumor metastases and cell-mediated immun
Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. VI. Similar specificity patterns of protective anti-tumor immunity in vivo and of cytolytic T cells in vitro
✍ K. Bosslet; V. Schirrmacher; G. Shantz 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 954 KB

## Abstract In an attempt to analyze mechanisms of immunity against tumor metastases, protective anti‐tumor immunity __in vivo__ was compared with cytotoxic T‐cell activity __in vitro__ in a well‐defined syngeneic tumor model system. The system consists of a chemically induced parental tumor cell l

Tumor metastases and cell-mediated immun
Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. V. Transfer of protective immunity with H-2 identical immune T cells from B10.D2 mice
✍ V. Schirrmacher 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 683 KB

## Abstract Evidence is presented for the existence of immune T lymphocytes which have protective activity __in vivo__ against the lethal effects of a metastasizing tumor. ESb, the metastasizing tumor cell line of our model system, which is highly malignant in syngeneic DBA/2 mice, can be rejected