Tumor Cell Variants Obtained by Mutagenesis of a Lewis Lung Carcinoma Cell Line: Immune Rejection by Syngeneic Mice
โ Scribed by Aline Van Pel, Myriam Georlette and Thierry Boon
- Book ID
- 123656111
- Publisher
- National Academy of Sciences
- Year
- 1979
- Tongue
- English
- Weight
- 830 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/70433
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## Abstract The development and growth of transplanted syngeneic Lewis lung carcinoma and their relation to aging was investigated. The Lewis lung carcinoma (3LL) is a metastasizing tumor which developed spontaneously in a C57BL/6J mouse. We found that the frequency and rate of tumor growth were in
Medium conditioned by the established lung tumor cell line A549 was used as a supplement to culture cells from primary solid lung tumors. Of 36 cases placed into culture, primary cells were obtained in 33 (91.7%). Of 29 cases in which subcultures were attempted, 18 (62.1%) were successful. Nine cell