Tubulin polymerization-promoting protein (TPPP/p25) is critical for oligodendrocyte differentiation

✍ Scribed by Attila Lehotzky; Pierre Lau; Natália Tőkési; Naser Muja; Lynn D. Hudson; Judit Ovádi

• Book ID: 102847927
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 528 KB
• Volume: 58
• Category: Article
• ISSN: 0894-1491
• DOI: 10.1002/glia.20909

✦ Synopsis

Abstract

TPPP/p25, a recently identified tubulin polymerization‐promoting protein (TPPP), is expressed mainly in myelinating oligodendrocytes of the CNS. Here, we show that TPPP/p25 is strongly upregulated during the differentiation of primary oligodendrocyte cells as well as the CG‐4 cell line. The microRNA expression profile of CG‐4 cells before and after induction of differentiation was established and revealed differential regulation of a limited subset of microRNAs. miR‐206, a microRNA predicted to target TPPP/p25, was not detected in oligodendrocytes. Overexpression of miR‐206 led to downregulation of TPPP/p25 resulting in inhibition of differentiation. Transfection of siRNAs against TPPP/p25 also inhibited cell differentiation and promoted cell proliferation, providing evidence for an important role of TPPP/p25 during oligodendrogenesis. These results support an essential role for TPPP/p25 in oligodendrocyte differentiation likely via rearrangement of the microtubule system during the process elongation prior to the onset of myelination. © 2009 Wiley‐Liss, Inc.