Development of a Tryptophan Auxotrophic
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J. Mukherjee; M. Menge; D. Hoischen; N. Grammel; E. Winterfeldt; U. Keller; Th.
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Article
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2002
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John Wiley and Sons
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English
β 74 KB
Four tryptophan dependent mutants of Claviceps purpurea 1029 N5 were derived by treatment with N-methyl-Nβ²-nitro-N-nitroguanidine (NTG) and protoplasting of the treated fungus. The poor alkaloid production of these mutants required a systematic optimisation of the production media. Malic acid was es