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Tryptophan phosphorescence of ribonuclease T1as a probe of protein flexibility

โœ Scribed by Margherita Gonnelli; Alessandro Puntoni; Giovanni Battista Strambini


Book ID
104982142
Publisher
Springer
Year
1992
Tongue
English
Weight
902 KB
Volume
2
Category
Article
ISSN
1053-0509

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## Abstract The introduction of disulfide crosslinks is a generally useful method by which to identify regions of a protein that are close together in space. Here we describe the use of disulfide crosslinks to investigate the structure and flexibility of a family of designed 4โ€helix bundle proteins