𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Tryptophan genes in Rhizobium—Their organization and their transfer to other bacterial genera

✍ Scribed by Johnston, A. W. B. ;Bibb, M. J. ;Beringer, J. E.

Book ID
104726278
Publisher
Springer
Year
1978
Tongue
English
Weight
774 KB
Volume
165
Category
Article
ISSN
0026-8925

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✦ Synopsis

R. leguminosarum trp alleles mapped by R68.45-mediated recombination were located in three distinct chromosomal regions. We isolated three derivatives of R68.45 that carried different trp genes of R. meliloti. Each of the plasmids suppressed all of the R. leguminosarum trp alleles in a particular region. The R-primes were transferred to strains of P. aeruginosa carrying mutations in different trp genes. The plasmid pAJ24JI suppressed trpA, B and F mutants, pAJ73JI suppressed trpC and D and pAJ88JI suppressed a trpE mutant. When the R-primes were transferred to E. coli trp strains they failed to suppress any trp mutants. A derivative of pAJ24JI was isolated which was able to suppress trpA and F mutants of E. coli.

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