Tryptophan-contained peptide-functional nanomaterials as general spectrofluorometric reagents for enzyme

Phenylglyoxal and glyoxal as fluorogenic

Phenylglyoxal and glyoxal as fluorogenic reagents selective for N-terminal tryptophan-containing peptides

✍ Eijiro Kojima; Yoshihito Ohba; Masaki Kai; Yosuke Ohkura 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 516 KB

Methods are described for the determination of N-terminal tryptophan-containing peptides in which phenylglyoxal and glyoxal are used as fluorogenic reagents. Phenylglyoxal and glyoxal react selectively with the peptides when heated in a strongly acidic medium in the presence of potassium hexacyanofe