An improved method for the isolation of
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Peter Bellemann; Rolf Gebhardt; Dieter Mecke
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Article
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1977
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Elsevier Science
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English
⚖ 512 KB
An improved nonperfusion method for the isolation of hepatic parenchymal cells using a modified technique of collagenaseihyaluronidase digestion with liver slices is described. The yield of viable cells was considerably increased (30 x IO6 cells/g of liver) by bubbling all solutions with carbogen (9