𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Truncated fragments in polymerase chain reaction-based DNA sequencing

✍ Scribed by Joakim Westberg; Anders Holmberg; Mathias Uhlén; Bertil Pettersson

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
198 KB
Volume
20
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Detection of HPV DNA in trichilemmomas b
Detection of HPV DNA in trichilemmomas by polymerase chain reaction
✍ Rohwedder, Angela; Keminer, Oliver; Hendricks, Carlo; Schaller, Jörg 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 269 KB 👁 2 views

Paraffin sections of 11 formalin-fixed trichilemthe genital HPVs (A); the epidermodysplasia verrucimomas were investigated for the presence of formis (EV)-associated HPVs (B); ungulate associated human papillomavirus (HPV) DNA by the polypapillomaviruses (C and D) and cutaneous HPVs (E) merase chain

Amplification of human genomic DNA seque
Amplification of human genomic DNA sequences with polymerase chain reaction using a single oligonucleotide primer
✍ Liuying Luo; Eleftherios P. Diamandis 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 157 KB

We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only

Quantitative DNA methylation analysis by
Quantitative DNA methylation analysis by fluorescent polymerase chain reaction single-strand conformation polymorphism using an automated DNA sequencer
✍ Hiromu Suzuki; Fumio Itoh; Minoru Toyota; Takefumi Kikuchi; Hideki Kakiuchi; Yuj 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB 👁 1 views

A novel DNA methylation assay technique, termed bisulfite single-strand conformation polymorphism (bisulfite-SSCP), is a combination of sodium-bisulfite modification and fluorescence-based polymerase chain reaction (PCR)-SSCP. After bisulfite treatment followed by PCR amplification, methylated and u

Mass Spectrometric Gene Diagnosis of One
Mass Spectrometric Gene Diagnosis of One-base Substitution from Polymerase Chain Reaction Amplified Human DNA
✍ Toshihiro Tsuneyoshi; Keiichiro Ishikawa; Yoshinori Koga; Yasuhiro Naito; Shozo 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 109 KB

One-base substitution has been detected on the polymerase chain reaction (PCR) products amplified from human mutated DNA for the first time by using mass spectrometry. PCR fragments of 52 base pairs were produced on a collagen gene of an osteogenesis imperfecta patient's heterozygous DNAs. The produ