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True reversion of a mutation in the chloroplast gene encoding the large subunit of ribulosebisphosphate carboxylase/oxygenase inChlamydomonas

โœ Scribed by Robert J. Spreitzer; Michele Rahire; Jean-David Rochaix


Publisher
Springer-Verlag
Year
1985
Tongue
English
Weight
246 KB
Volume
9
Category
Article
ISSN
0172-8083

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โœฆ Synopsis


The ribulosebisphosphate carboxylase/oxygenase-defective Chlarnydomonas mutant, 10-6C, was the first mutant to be physically defined in chloroplast DNA. In this report, a photosynthesis-competent revertant of the 10-6C mutant has been found to result from true reversion within the chloroplast large-subunit gene. This result supports the original assignment of the 10-6C mutation within the large-subunit gene.


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Heteroplasmic suppression of an amber mu
โœ Robert J. Spreitzer; Chris J. Chastain ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 543 KB

The 18-5B and 18.7G mutants of Chlamydomonas reinhardtii lack ribulosebisphosphate carboxylase/ oxygenase holoenzyme and contain nonsense mutations in the chloroplast gene that encodes the protein's large s ubunit. Spontaneous revertants of the 18-5B opal (UGA) mutant were found to be heteroplasmic