Trophoblast glycoprotein recognised by monoclonal antibody 5T4 maps to human chromosome 6q14–q15
✍ Scribed by J. M. Boyle; K. H. Grzeschik; P. R. Heath; J. E. Morten; P. L. Stern
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 468 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
Human x rodent hybrids were stained by indirect immunofluorescence with 5T4, a murine monoclonal antibody that recognises a 72 kdalton glycoprotein expressed by human trophoblasts and a very restricted range of adult tissues; they were analysed by flow cytometry. Concordance analysis supported by segregation data allowed assignment of the gene controlling glycoprotein expression (M6P1) to chromosome 6. Similar analysis with translocation hybrids gave a regional assignment to 6q14-q15, M6P1 is distinct from NT5, coding for 5' nucleotidase, which maps to the same region.