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Trophectoderm cell subpopulations in the periimplantation mouse blastocyst

✍ Scribed by Chávez, Daniel J. ;Enders, Allen C. ;Schlafke, Sandra

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
472 KB
Volume
231
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Trophectoderm of the preimplantation mouse blastocyst is composed of two cell subpopulations relative to their proximity to the inner cell mass. The polar trophectoderm overlying the inner cell mass proliferates to form the ectoplacental cone, and the mural trophectoderm endoreplicates and gives rise to giant cells. We examined specific differences in the two trophectoderm cell populations using a lectin (Dolichos biflorus) to detect cell surface characteristics and a simple sugar (D‐Gal) to detect differences in incorporation. During the first day of delayed implantation, the mural trophectoderm presented twice as many lectin binding sites as did the polar trophectoderm. The mural trophectoderm of both nondelaying and delayed implantation blastocysts showed a greater rate of incorporation of the tritiated sugar by presenting more reduced silver grains in radioautograms. These results indicate that the mural trophectoderm and polar trophectoderm are two distinct cell types in the periimplantation blastocyst.

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