Tritium labeling of full-length small interfering RNAs
✍ Scribed by Jesper Christensen; François Natt; Jürg Hunziker; Joel Krauser; Hendrik Andres; Piet Swart
- Book ID
- 112132917
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- French
- Weight
- 365 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0022-2135
No coin nor oath required. For personal study only.
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## Abstract Small interfering RNAs (siRNAs), a class of macromolecules constituted by the association of two single‐stranded ribonucleic acids of short sequences, have been labelled with the positron‐emitter fluorine‐18 (__T__~1/2~: 109.8 min). The strategy involves (1) prosthetic conjugation of a