Triterpenes from the Fungus Poria cocos and Their Inhibitory Activity on Nitric Oxide Production in Mouse Macrophages via Blockade of Activating Protein-1 Pathway
✍ Scribed by Tian-Ge Cai; Yu Cai
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 395 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1612-1872
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✦ Synopsis
Abstract
Two new triterpenes, 29‐hydroxydehydrotumulosic acid (1) and 29‐hydroxydehydropachymic acid (2), together with six known compounds, dehydropachymic acid (3), dehydrotumulosic acid (4), 29‐hydroxypolyporenic acid C (5), polyporenic acid C (6), tumulosic acid (7), and pachymic acid (8), were isolated from the dried sclerotia of Poria cocos. In the in vitro bioassays, these isolated compounds reduced, in a dose‐dependent manner, nitric oxide (NO) production from lipopolysaccharide (LPS)‐induced RAW 264.7 cells, with compounds 5 and 6, the IC~50~ values of which were 16.8±2.7 and 18.2±3.3 μM, respectively, exhibiting the greatest inhibition activity. Further Western blot analysis conducted on cells pre‐treated with compounds 5 and 6, and luciferase assays on activator protein 1‐dependent gene expression revealed that the inhibited NO release was attributed to the reduced expression of iNOs (=inducible NO synthase) enzymes, which might be regulated via the blockade of activator protein‐1 signaling pathway.