✦ LIBER ✦

Tris(2,2′-bipyridyl) ruthernium(II)-mediated photoinduced electron transfer of engineered cytochrome P450 1A2

✍ Scribed by Ryosuke Nakano; Hideaki Sato; Toru Shimizu

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 502 KB
Volume: 32
Category: Article
ISSN: 1011-1344
DOI: 10.1016/1011-1344(95)07230-6

No coin nor oath required. For personal study only.

✦ Synopsis

In this study, it is shown that an electron from photoreduced tris(2,2'-bipyridyl)Ru2+ ion reaches the haem iron of engineered wild-type cytochrome P450 1A2 (P450 1A2) with an electron transfer rate of 6.04 x 10(-3) min(-1). The electron transfer rate, 4.05 x 10(-2) min(-1), of a His163Glu mutant, which has a redox potential 40 mV lower than that of the wild type, is more than sixfold faster than that of the wild type. The photoinduced electron transfer rates of the present system are strongly influenced by detergents, cholic acid and Emulgen 913. We discuss the intermolecular and intramolecular electron transfer mechanism of the P450 1A2 system based on the kinetic data.

📜 SIMILAR VOLUMES

Use of genetically engineered Salmonella

Use of genetically engineered Salmonella typhimurium OY1002/1A2 strain coexpressing human cytochrome P450 1A2 and NADPH-cytochrome P450 reductase and bacterial O-acetyltransferase in SOS/umu assay

✍ Pramod Aryal; Takao Terashita; F. Peter Guengerich; Tsutomu Shimada; Yoshimitsu 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 95 KB 👁 1 views

Photoinduced Electron Transfer in a Tris

Photoinduced Electron Transfer in a Tris(2,2′-bipyridine)-C60-ruthenium(II) Dyad: Evidence of Charge Recombination to a Fullerene Excited State

✍ Michele Maggini; Dirk M. Guldi; Simonetta Mondini; Gianfranco Scorrano; Francesc 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB

Human hepatic and renal microsomes, cyto

Human hepatic and renal microsomes, cytochromes P450 1A1/2, NADPH:Cytochrome P450 reductase and prostaglandin H synthase mediate the formation of aristolochic acid-DNA adducts found in patients with urothelial cancer

✍ Marie Stiborová; Eva Frei; Petr Hodek; Manfred Wiessler; Heinz H. Schmeiser 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 French ⚖ 139 KB

## Abstract Aristolochic acid (AA), a naturally occurring nephrotoxin and carcinogen, has been associated with the development of urothelial cancer in humans. Understanding which human enzymes are involved in AA activation and/or detoxication is important in the assessment of an individual's suscep

A New Mechanism for Ethanol Oxidation Me

A New Mechanism for Ethanol Oxidation Mediated by Cytochrome P450 2E1: Bulk Polarity of the Active Site Makes a Difference

✍ Yong Wang; Chuanlu Yang ; Hongming Wang; Keli Han ; Sason Shaik 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 292 KB 👁 1 views

Intratumoral activation of cyclophospham

Intratumoral activation of cyclophosphamide by retroviral transfer of the cytochrome P450 2B1 in a pancreatic tumor model. Combination with the HSVtk/GCV system

✍ Meritxell Carrió; Joana Visa; Anna Cascante; Xavier Estivill; Cristina Fillat 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB

## Abstract ## Background Pancreatic cancer is one of the most aggressive human tumors and the development of new therapeutic approaches is particularly urgent since current therapies are not effective. The use of pro‐drug‐activating genes is a possible approach for cancer gene therapy. ## Method