Triple Resonance Solid State NMR Experiments with Reduced Dimensionality Evolution Periods

✍ Scribed by Nathan S Astrof; Charles E Lyon; Robert G Griffin

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 89 KB
• Volume: 152
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.2001.2406

✦ Synopsis

Two solid state NMR triple resonance experiments which utilize the simultaneous incrementation of two chemical shift evolution periods to obtain a spectrum with reduced dimensionality are described. The CO N CA experiment establishes the correlation of 13 C i-1 to 13 Cα i and 15 N i by simultaneously encoding the 13 CO i-1 and 15 N i chemical shifts. The CA N COCA experiment establishes the correlation 13 Ca i and 15 CO i to 13 Cα i-1 and 15 N i-1 within a single experiment by simultaneous encoding of the 13 Cα i and 15 N i chemical shifts. This experiment establishes sequential amino acid correlations in close analogy to the solution state HNCA experiment. Reduced dimensionality 2D experiments are a practical alternative to recording multiple 3D data sets for the purpose of obtaining sequence-specific resonance assignments of peptides and proteins in the solid state.