Triple Helices of Optimally Capped Duplex DNA (σ-DNA) with Homopyrimidine DNA and RNA at Neutral pH

✍ Scribed by Willi Bannwarth; Patrick Iaiza

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: German
• Weight: 122 KB
• Volume: 82
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/(sici)1522-2675(19991110)82:11<1806::aid-hlca1806>3.0.co;2-l

✦ Synopsis

Dedicated to Prof. Dr. Frank Seela on the occasion of his 60th birthday An optimally capped duplex DNA (s-DNA) was synthesized in which the cytosines in the homopyrimidine strand of s-DNA 1 were replaced by 5-methylcytosine leading to s-DNA 2. Although only involving 13 base pairs, this modification resulted in very high melting temperatures above 908. In addition, s-DNA 2 was able to form triple helices with the corresponding homopyrimidine DNA or RNA even at neutral pH. This opens up the possibility to use s-DNA in a triple-helix approach to modulate gene expressions on the level of the translation process.