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Trichodermin, a possible inhibitor of the termination process of protein synthesis

✍ Scribed by Mary E. Stafford; Calvin S. McLaughlin

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
639 KB
Volume
82
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The mode of action of the antibiotic, trichodermin, on yeast cells has been investigated. Trichodermin specifically inhibits protein synthesis and, during the in vivo inhibition of protein synthesis, ribosomes remain in polyribosomes rather than shifting to monoribosomes. This observation suggests that trichodermin inhibits either an elongation step or a termination step of protein biosynthesis. These two possibilities were distinguished by comparing the action of trichodermin with that of cycloheximide, a known elongation inhibitor, upon the reformation of polyribosomes during recovery from a block in polypeptide chain initiation. Cycloheximide slows the recovery of polyribosomes from monoribosomes following a block in polypeptide chain initiation whereas trichodermin enhances the recovery of polyribosomes. This observation is interpreted to mean that trichodermin primarily inhibits the termination step of protein biosynthesis.

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