Trennung und Charakterisierung von β-Carotin-Isomeren

✍ Scribed by Max Vecchi; Gerhard Englert; Res Maurer; Vladimir Meduna

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: German
• Weight: 699 KB
• Volume: 64
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19810640836

✦ Synopsis

Separation and Characterization of the cis‐Isomers of β,β‐Carotene

A stable HPLC. system is described allowing the excellent separation of 11 different cis‐isomers of β,β‐carotene from the all‐trans compound. The system is applied to the analysis of cis/trans mixtures obtained from plant extracts and by photoisomerization of the all‐trans isomer. Al~2~O~3~ is used as the stationary phase while hexane with controlled H~2~O content is utilized as the mobile phase. With the aid of the optimum conditions 8 sufficiently stable cis isomers were isolated and their structures shown to be the 9‐, 13‐ and 15‐cis, the 9,9′‐, 9, 13‐, 9, 13′‐ and 13,13′‐di‐cis and, tentatively, the 9,13,13′‐tri‐cis β,β‐carotenes by application of 270‐MHz‐FT.‐^1^H‐NMR. spectroscopy.