Trehalase from Sclerotinia sclerotiorum

✍ Scribed by Wang, Sy-Ying C. ;Tourneau, Duane

• Book ID: 104769875
• Publisher: Springer-Verlag
• Year: 1972
• Weight: 420 KB
• Volume: 87
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00424883

✦ Synopsis

Cell-free extracts of mycelia and sclerotia ofSclerotinia sclerotiorum (Lib.) D By. grown on synthetic liquid medium with various carbon sources conrained trehalase (a,a'-glucoside 1-glucohydrolase; EC 3.2.1.28) activity. The enzyme was not usually detected in the culture filtrate. Treatment with ammonium sulfate or MnSO 4 and alumina resulted in a 2-to 3-fold purification. The optimum pH (5.0), Km with trehalose (1.7• 10 -3 M) and other properties are within the range reported for trehalase from other fungi.

Trehalose is found in mycelia and sclerotia of Sclerotinia sclerotiorum