Phage MudII301 was used to isolate new periplasmic-leaky mutants of Escherichia coli K12 carrying an lkyB-lacZ gene fusion. The properties of strain JC2299 carrying the lkyB-2299 insertion mutation were identical to those of strain JC207 carrying the previously described lkyB-207 mutation. The LkyB-
✦ LIBER ✦
Treatment with DNA-damaging agents increases expression of polA`-'lacZ gene fusions in Escherichia coli K-12
✍ Scribed by Wandt, G. ;Kubis, S. ;Quiñones, A.
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 169 KB
- Volume
- 254
- Category
- Article
- ISSN
- 0026-8925
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We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (darn-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of fl-galactosidase in darn-strains than in isogen