BACKGROUND: In this work, the feasibility of biodegradation and the removal performance of sole and mixed odorous vapors, such as dimethyl disulfide (DMDS), methyl phenyl sulfide (MPS), and ethanethiol (EtSH) in an EtSH-acclimated biotrickling filter seeded with commercially available B350 microorganisms, were investigated. RESULTS: Removal efficiencies (REs) for DMDS as a sole substrate were evaluated under different inlet concentrations and empty bed residence times (EBRT), 100% RE was achieved at concentration below 0.4 g m -3 at EBRT 110 s. In addition, 100% RE was obtained for binary EtSH and DMDS (3 : 2) at the same EBRT. According to the Michaelis-Menten type kinetic equation, the maximum removal rates (V max ) were calculated as 28.7 and 13.9 g m -3 h -1 for DMDS and MPS as sole substrate, respectively, while V max was 22.1 and 10.1 g m -3 h -1 for DMDS and MPS in the presence of EtSH and EtSH-DMDS mixture, respectively. After 5 and 20 days starvation, the re-acclimation times were only 2 and 8 days, respectively, for the binary system. An EtSH : DMDS : MPS (3 : 2 : 1) ternary mixture was removed efficiently by the rebooted system after starvation. CONCLUSION: The proposed system can be applied to cost-effectively decompose a mixture of volatile organic sulfide compounds at pilot scale.