Treatment of amylomaize starch granules with urea: comparison with normal maize starch
โ Scribed by Tadashi Baba; Seong-Jin Kim; Yuji Arai
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 295 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
Two of the main features of starch granules are their insolubility in water and their very low susceptibility to the action of various enzymes. These properties are generally considered to be due to the formation of a stable hydrogen-bonded structure within the granules. Treatment with boiling water, alkali, acid, methyl sulfoxide, and urea are usually required to denature starch granules'. The interior of the starch granules can be solubilised by treatment with a high concentration of ureaze5, and it has been suggested *-'that the solubilised part and the residual outside-layer are equivalent to the amylose and amylopectin fractions, respectively. in the original granules.
Amylomaize starch has a high content of amylose', and its properties and
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A method of separating amylose and amylopectin is based on proteolysis of the precipitated concanavalin A-amylopectin complex. The molecular-size distributions, together with the i,,, and A,,, of the iodine complexes of fractions obtained by gel chromatography, indicated efficient separation ofamylo