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Trapping method of antibodies on surfaces of polymerizing discs for enzyme immunoassay

โœ Scribed by Alumanda M. Dela Rosa; Minoru Kumakura


Book ID
107710204
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
730 KB
Volume
312
Category
Article
ISSN
0003-2670

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Two methods of preparing enzyme-antibody conjugates were evaluated. High yields of conjugate were obtained with both methods. The first procedure utilizes the homobifunctional crosslinking reagent N,N'-o-phenylenedimaleimide. Sulfydryl residues were introduced into second antibodies by reaction wit