Transverse-Relaxation-Optimized (TROSY) Gradient-Enhanced Triple-Resonance NMR Spectroscopy

✍ Scribed by J.Patrick Loria; Mark Rance; Arthur G. Palmer III

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 76 KB
• Volume: 141
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1891

✦ Synopsis

Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO-TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a 15 N spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer.