Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm

✍ Scribed by Gloria S. Kwon; Anna-Katerina Hadjantonakis

• Book ID: 102156253
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 368 KB
• Volume: 47
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/dvg.20556

✦ Synopsis

Abstract

Laser scanning confocal images of embryonic day (E) 5.5 (left pair) and 6.5 (right pair) Ttr::RFP ; Afp::GFP transgenic mouse embryos expressing red fluorescent protein (RFP) and green fluorescent protein (GFP) reporters in the visceral endoderm. Data are depicted as single optical sections of merged GFP and RFP channels (lefthand image of each pair), demonstrating that these reporters are visceral endoderm‐specific with fluorescence restricted to the surface of the embryo. 3D reconstructions of confocal z‐stacks reveal reporter localization throughout the visceral endoderm (righthand image of each pair). See the paper by Kwon and Hadjantonakis in this issue.